In this review Mirko Francesconi and Ben Lehner describe some of the computational methods that can be applied to identify and interpret sources of variance in gene expression in whole organisms, organs, tissues or single cells.
In this article Pengelly et al. show that H2Aub is essential for viability and required for efficient histone H3 Lys27 trimethylation by PRC2 early in embryogenesis. Furthermore they demonstrate that PRC1 represses canonical target genes independently of H2Aub.
In this article Baymaz et al. argue that integration of various state-of-the-art technologies, addressing different aspects of transcriptional regulation, is essential to unravel this apparent biological versatility of 'co-repressor' complexes
In this paper Palayret et al. demonstrate 3D sectioning with single-molecule super-resolution microscopy by making use of the fitting information that is usually discarded to reject fluorophores that emit from above or below a virtual-'light-sheet'.