Developing a global understanding of the PRC and NuRD complexes in stem cell differentiation and in disease

Genetic code expansion for multiprotein complex engineering - Berger Lab (2016)

We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, 'MultiBacTAG', (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure–function studies of novel eukaryotic complexes using single-molecule Förster resonance energy transfer as well as site-specific crosslinking strategies.



The data sets generated during this study are available in the European Nucleotide Archive (accession code PRJEB12116).


More information also at: http://www.bristol.ac.uk/biochemistry/news/2016/multibac-tag.html