Developing a global understanding of the PRC and NuRD complexes in stem cell differentiation and in disease

DPY30 regulates pathways in cellular senescence through ID protein expression — Di Croce and collaborator labs (2013)

Elisabeth Simboeck, Arantxa Gutierrez, Luca Cozzuto, Malte Beringer, Livia Caizzi, William M Keyes, and Luciano Di Croce 

Cellular senescence is an intrinsic defense mechanism to various cellular stresses: while still metabolically active, senescent cells stop dividing and enter a proliferation arrest. Here, we identify DPY30, a member of all mammalian histone H3K4 histone methyltransferases (HMTases), as a key regulator of the proliferation potential of human primary cells. Following depletion of DPY30, cells show a severe proliferation defect and display a senescent pheno- type, including a flattened and enlarged morphology, elevated level 

of reactive oxygen species (ROS), increased SA-b-galactosidase activity, and formation of senescence- associated heterochromatin foci (SAHFs). While DPY30 depletion leads to a reduced level of H3K4me3-marked active chromatin, we observed a concomitant activation of CDK inhiitors, including p16INK4a, independent of H3K4me3. ChIP experiments show that key regulators of cell-cycle progression, including ID proteins, are under direct control of DPY30. Because ID proteins are negative regulators of the transcription factors ETS1/2, depletion of DPY30 leads to the transcriptional activation of p16INK4a by ETS1/2 and thus to a senescent-like phenotype. Ectoptic re-introduction of ID protein expres- sion can partially rescue the senescence-like phenotype induced by DPY30 depletion. Thus, our data indicate that DPY30 controls proliferation by regulating ID proteins expression, which in turn lead to senescence bypass. 

EMBO J. (2013) Jul 19. doi: 10.1038/emboj.2013.159


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